Background: Because of population growth, limited access to fresh water resources, the need to use bottled water, controlling microbial quality of  bottled water is important.

Materials and Methods: Microbiological quality of 24 brands of bottled water available in the town markets of Iran was studied Random. Samples were collected in summer and autumn, 2012. In each season, we collected two samples for each brand. In order to analyze Total coliforms, E-Coli, and HPC, MPN and Plate Count Methods were used. Data analysis was processed by SPSS software.

Results: Total coliforms were 2 MPN/100CC in two brands S18 and S20. Increased HPC levels were also observed in all brands. pH level of 6% from bottled waters were higher than the standard. Average of turbidity was 0.232 and 0.228 at the autumn and summer, respectively.

Conclusion: the heterotrophic microorganisms were present in 100% of the samples. Total coliforms were also found in 12% of the samples. None of the samples contained E-Coli.