Backgrounds and Objectives: Ice contamination comes from different ways. Considering the importance of hygiene of ice the purpose of this study was to investigate microbial contamination of the ice-making factories and sales centers in Ghaemshahr.

Materials and Methods: In this Cross - sectional study, 6 & 20 samples were collected randomly from 2 ice-making factory and sales centers. Six water samples were taken from the factory. They were then transferred to lab. Every ice sample was tested before and after washing with water. Then Total Coli forms and Escherichia coli were determined.

Results: The results showed that water sample is without pollution. The sample of the ice-making unit A isn^´t safe. Unit B isn^´t. E.Coli contamination but has 2 cases of coliform contamination. The ice sale place has (before & after Washing with water) respectively 95% -80%  E.Coli contamination and 100% total coliform.

Conclusion: There isn^´t any relationship between water quality and microbial contamination .Sanitary conditions Unit A is better than B. Personal hygiene, work tools and Building had a positive effect in contamination of ice. The results suggest that more attention of health system to control and prevent microbial contamination of ice is necessary.