Background: Microorganisms in hospitals are potential sources of infection to patients and staff.   Bioaerosol exposure is associated with a vast range of adverse health effects including infectious diseases, acute toxic effects, allergy and cancer. This study aimed to survey the type and density of bioaerosols in ambient air of different parts of educational hospitals Gorgan 2012.

Materials and Methods: In this cross-sectional study, different wards of Gorgan educational hospitals including dialysis, patient room and ICU were investigated. Totally, 81 air samples were taken based on NIOSH standard method. Saborad Dextrose agar and Nutrient agarculture medium was used passive method media. Samples collected were shipped to lab and incubated for 48 hours. Finally Concentration was determined in cfu/m2/hr.

Results: The diversity and density of Bioaerosoles the highest and lowest densities were in dialysis (30.65%) and operating rooms (19.55%) wards of panjom azar hospital and surgical women (23.35%) and operating rooms(15.35%) wards of  Sayyad Shirazi hospital. The most common fungi and bacteria isolated from air hospitals were Staphylococcus epidermisand (41%) and penicillium (23.45%).

Conclusion: According to the results of the chi-square test for detection of bacterial contamination of surfaces and equipment in the hospitals studied did not show a significant statistical difference (p-value<0.5) and the highest percentage of bacterial contamination of December is the fifth hospital. Also compared fungal infection in two hospitals showed that there is a significant statistical difference (p-value 0.000) and the highest percentage of fungal infections of December is the fifth hospital.