Background & Objectives: Nowadays, antibiotic residue presence in environment is a world concern, so there discharge control necessity is unavoidable.  The objective of this research was the study of Tetracycline removal from aqueous solution by Nano Zero Valent Iron/UV/H₂O₂ process.

Methods: This research was a lab-scale experimental study that conducted in laboratory temperature. Synthetics wastewater with concentration of 15, 30, 45, and 60 mg/l prepared from stock solution (500 mg/l). The effect of operational parameters such as pH, nano zero valent iron concentration, Tetracycline antibiotic concentration , UV Radiation , H₂O₂  concentration and contact time determined by one at  a time method. Tetracycline antibiotic concentrations determined by UV-HPLC equipment and COD variation analyzed during the study. 

Results: The results showed that tetracycline antibiotics removal efficiency in optimum conditions and initial concentrations of 15, 30, 45, and 60 mg/l were 85, 94, 100, and 78 percent, respectively. So COD removals were 35.4, 55.58, 60.3, and 33.85.

Conclusion: Nano Zero Valent Iron/UV/H₂O₂ process is a efficient process to the removal of tetracycline antibiotic from the wastewater. So efficiency of this method can be evaluated in removal of other resistant antibiotics to biological treatment.