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:: Volume 10, Issue 1 (12-2022) ::
jehe 2022, 10(1): 72-82 Back to browse issues page
Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa
Bahram Kamarehie , Fatemeh Farash Beiranvand , Mohsen Mohammadi , Mohammad Amin Karami *
Environmental Health Research Center, Lorestan University of Medical sciences, Khorramabad, Iran
Abstract:   (236 Views)
Background and objective:  Increasing antibiotic resistance in most bacteria has led to the development of natural antimicrobial compounds. The purpose of this study was to investigate the disinfection effect of Ajowan essential oil (EO) on the standard strain of Pseudomonas aeruginosa
Methods: In this study, the essential oil was prepared by distillation of Ajowan. The components of the essential oil were separated and identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Then the antibacterial effects of two combinations including Ajowan EO +dimethyl sulfoxide (DMSO) and glutaraldehyde 2% on standard strain of Pseudomonas aeruginosa were evaluated and determined using dilution broth and disk diffusion methods
Results: The results of the study showed that the most component of the essential oil consist of: Thymol (50%), P-cymene (23.6%), and Terpinen (20.3%). The mean values of MIC in the antibacterial substances (Ajowan EO+DMSO,   Glutaraldehyde 2%) were 83 and 125µg/mL respectively. The mean values of MBC for abovementioned substances were 125 and 166 µg/mL respectively. The mean diameter of the inhibition zone in the MIC concentration was 11and 12.66 mm respectively.
Conclusion: According to the obtained results, Essential oils Ajowan has similar effect of glutaraldehyde 2% on Pseudomonas aeruginosa. It may be a possible substitute to glutaraldehyde in hospital disinfection purposes.
Keywords:   Disinfectant, Ajowan, Nosocomial infection
Full-Text [PDF 886 kb]   (173 Downloads)    
Type of Study: Research | Subject: Special
Received: 2022/02/22 | Accepted: 2023/03/5 | Published: 2023/07/10
References
1. Relf MV, Mekwa J, Chasokela C, et al. Essential nursing competencies related to HIV and AIDS. Journal of the Association of Nurses in AIDS Care 2011;22(1): e5-e40. [DOI:10.1016/j.jana.2010.07.007] [PMID] []
2. Campos GB, Souza SG, Lobão TN, et al. Isolation, molecular characteristics and disinfection of methicillin-resistant Staphylococcus aureus from ICU units in Brazil. New Microbiologica 2012;35(2): 183-90.
3. Zazouli MA, Homayoun nasab Langroodi M, Ahanjan M, et al. Efficiency of some disinfectants (Cidex, Deconex, and Creolin) against E. Coli. Journal of Mazandaran University of Medical Sciences 2015;24(122): 137-46.
4. Ducel G, Fabry J, Nicolle L. Prevention of hospital acquired infections: a practical guide. Prevention of hospital acquired infections: a practical guide 2002(Ed. 2).
5. Dadmanesh M, Dormanesh B, GHASEMZADEH S, et al. Evaluation of nosocomial urinary tract infection in the intensive care unit patients at Tehran 501 hospital during 2007. 2008.
6. Mehdi Q, Reza R, Nematollah J, et al. A Study on the Prevalence of Nosocomial Infections in ICU Patients Admitted at Baqyiatallah Hospital. Journal of Ilam University of medical sciences 2008;16(1): 1-6.
7. Doosti M, Faghihi MHO, Ramazani A, Saini MR. Comparison of Conventional Culture Methods and Polymerase Chain Reaction (PCR) for Specific Detection of Pseudomonas Aeruginosa. Journal of Isfahan Medical School 2012;30(192).
8. Velazquez-Meza ME, Hernández-Salgado M, Sánchez-Alemán MA. Evaluation of the antimicrobial activity of a super oxidized solution in clinical isolates. Microbial Drug Resistance 2015;21(4): 367-72. [DOI:10.1089/mdr.2014.0266] [PMID]
9. McDonnell G, Russell AD. Antiseptics and disinfectants: activity, action, and resistance. Clinical microbiology reviews 1999;12(1): 147-79. [DOI:10.1128/CMR.12.1.147] [PMID] []
10. Aali E, Mahmoudi R, Kazeminia M, et al. Essential oils as natural medicinal substances. Tehran University Medical Journal TUMS Publications 2017;75(7): 480-9.
11. Eblagh N, Fateh E, Farzane M, Osfuri M. Effect of Cattle Manure Application, Phosphate Solubilizing Bacteria and Different Phosphrous Levels on Yield and Essence Components of Trachyspermum Ammi L. JOURNAL OF AGRICULTURAL SCIENCE AND SUSTAINABLE PRODUCTION 2014;23(4.1): 1-15.
12. Haghiroalsadat F, Azhdari M, Oroojalian F, et al. The Chemical Assessment of Seed Essence of Three Native Medicinal Plants of Yazd Province (Bunium Premium, Cuminum Cyminum, Trachyspermum Copticum) and the Comparison of Their Antioxidant Properties. SSU_Journals 2015;22(6): 1592-603.
13. Fazeli-nasab B, Fooladvand Z. A review on Iranian Carum copticum (L.): Composition and biological activities. European J Med Plants 2016;12(1): 1-8. [DOI:10.9734/EJMP/2016/17584]
14. MURTHY PS, Borse BB, Khanum H, Srinivas P. Inhibitory effects of Ajowan (Trachyspermum ammi) ethanolic extract on A. ochraceus growth and ochratoxin production. Turkish Journal of Biology 2009;33(3: 211--7). [DOI:10.3906/biy-0805-13]
15. Fazeli-nasab B, Fooladvand Z. A review on Iranian Carum copticum (L.): Composition and biological activities. European Journal of Medicinal Plants 2016: 1-8. [DOI:10.9734/EJMP/2016/17584]
16. Rossolini G, Mantengoli E. Treatment and control of severe infections caused by multiresistant Pseudomonas aeruginosa. Clinical Microbiology and infection 2005;11: 17-32. [DOI:10.1111/j.1469-0691.2005.01161.x] [PMID]
17. Cigana C, Lore NI, Bernardini ML, Bragonzi A. Dampening host sensing and avoiding recognition in Pseudomonas aeruginosa pneumonia. Journal of Biomedicine and Biotechnology 2011;2011. [DOI:10.1155/2011/852513] [PMID] []
18. Bauer A, Kirby W, Sherris JC, turck. turck, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American journal of clinical pathology 1966;45(4): 493. [DOI:10.1093/ajcp/45.4_ts.493] [PMID]
19. Hasanvand T, Mohammadi M, Abdollahpour F, et al. A comparative study on antibacterial activity of carvacrol and glutaraldehyde on Pseudomonas aeruginosa and Staphylococcus aureus isolates: an in vitro study. Journal of Environmental Health Science and Engineering 2021;19: 475-82. [DOI:10.1007/s40201-021-00620-1] [PMID] []
20. Goudarzi GR, Saharkhiz M, Sattari M, Zomorodian K. Antibacterial activity and chemical composition of Ajowan (Carum copticum Benth. & Hook) essential oil. Journal of Agricultural Science and Technology 2011;13(2): 203-8.
21. Srivastava M, Baby P, Saxena A. GC-MS investigation and antimicrobial activity of the essential oil of Carum copticum Benth & Hook. Acta Alimentaria 1999;28(3): 291-5. [DOI:10.1556/AAlim.28.1999.3.8]
22. Bahmani M, Khaksarian M, Rafieian-Kopaei M, Abbasi N. Overview of the therapeutic effects of Origanum vulgare and Hypericum perforatum based on Iran's ethnopharmacological documents. Journal of Clinical and Diagnostic Research 2018;12(7) [DOI:10.7860/JCDR/2018/34177.11728]
23. Amiri A, Jomehpour N. Evaluation the Effect of Anti bacterial of Ferula assa-foetida L, Carum copticum, Mentha piperita L Hydroalcoholic Extract on Standard Sensitive and Methicillin-Resistant Staphylococcus aureus, Escherichia coli O157H7 and Salmonella typhimurium. 2016. [DOI:10.18869/acadpub.sjimu.24.2.72]
24. Goudarzi GR, Saharkhiz M, Sattari M, Zomorodian K. Antibacterial activity and chemical composition of Ajowan (Carum copticum Benth. & Hook) essential oil. 2011.
25. Khosravipour Sima, Roya R-D. Antibacterial activity of Carum copticum essential oil against Pectobacterium carotovorum subsp. carotovorum and Escherichia coli in nutrient broth medium. Research in Plant Pathology 2016;3(2): 43-57.
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Kamarehie B, Farash Beiranvand F, Mohammadi M, Karami M A. Evaluation of disinfection effect of Ajowan on Pseudomonas aeruginosa. jehe 2022; 10 (1) :72-82
URL: http://jehe.abzums.ac.ir/article-1-904-en.html


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Volume 10, Issue 1 (12-2022) Back to browse issues page
مجله مهندسی بهداشت محیط Journal of Environmental Health Enginering
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